Leica SP8 Confocal Microscope Specifications

Solid lasers

405nm(≥50mW); 448nm(≥40mW); 488 (≥20mW);514nm(≥20mW);552nm(≥20mW)

Microscope

Stand

Inverted Leica DMI6000B

Objectives

10×0.4 (dry), 20×0.7 (dry),40×0.85(dry), 63×1.20(WI),63×1.40(oil)

FL filter cubes

DAPI (Ex 340-380nm, DM 400, LP425nm)

GFP (Ex 450-490nm, DM510, Em LP515nm,)

TRITC (Ex 537/45nm, DM580, Em LP590nm)

Near Infrared (Ex 620/60nm, DM660, Em 700/75nm)

Scan module

Scan mode

x, y, z, λ, t, A, I combination with point, line and area scanning

Scan speed

7 fps@512×512 pixels for regular and 28fps@512×512 pixels for resonant scanning

Detector

1 PMT and 2 Hyd for fluorescence channel; 1 PMT for transmitted light

Software

Windows 7 64 bit, Leica X Version:1.1.0.12420

Application

BF; DIC; Pol,multi-spectrum fluorescence imaging; 3D imaging; FRAP; FRET; Live-cell imaging